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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

SNF2H Antibody #13453

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Filter:
  • WB
Western Blotting Image 1: SNF2H Antibody
Western blot analysis of extracts from various cell lines using SNF2H Antibody.
This product is discontinued

Inquiry Info. # 13453

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Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 125
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SNF2H Antibody recognizes endogenous levels of total SNF2H (SMARCA5) protein. This antibody does not cross-react with SMARCA1 protein.

Species Reactivity:

Human, Monkey

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Bovine, Dog, Pig, Horse, Guinea Pig

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala65 of human SNF2H (SMARCA5) protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Sucrose nonfermenting 2 homolog (SNF2H, SMARCA5) is one of two orthologs of the ISWI (imitation switch) ATPases encoded by the mammalian genome (1). SNF2H is part of the SNF2 family of chromatin remodeling factors that use ATP hydrolysis to catalyze biochemical reactions in several mammalian chromatin-remodeling complexes, including ACF1, RSF1, CHRAC, NoRC, WSTF, and WCRF180 (2). Research studies show that SNF2H is crucial for chromatin organization, DNA damage response, and differentiation (1-7). The SNF2H helicase facilitates DNA damage repair by actively moving nucleosomes for DNA damage response (DDR) proteins to effectively associate with damaged regions (3). Additional studies show that repair of double stranded breaks (DSBs) significantly decreases in the absence of SNF2H (3), and these cells become highly sensitive to DNA damage caused by x-rays and chemical treatments inducing DSBs (4,5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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