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SNF2H (D4W6N) Rabbit mAb #38410

Filter:
  • WB
Western Blotting Image 1: SNF2H (D4W6N) Rabbit mAb
Western blot analysis of extracts from K-562, HCT 116, and HeLa cell lines using SNF2H (D4W6N) Rabbit mAb.

To Purchase # 38410

Cat. # Size Qty. Price
38410T 20 µl
$153
38410S 100 µl
$339

Product Specifications

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 125
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SNF2H (D4W6N) Rabbit mAb recognizes endogenous levels of total SNF2H protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala65 of human SNF2H protein.

Background

Sucrose nonfermenting 2 homolog (SNF2H, SMARCA5) is one of two orthologs of the ISWI (imitation switch) ATPases encoded by the mammalian genome (1). SNF2H is part of the SNF2 family of chromatin remodeling factors that use ATP hydrolysis to catalyze biochemical reactions in several mammalian chromatin-remodeling complexes, including ACF1, RSF1, CHRAC, NoRC, WSTF, and WCRF180 (2). Research studies show that SNF2H is crucial for chromatin organization, DNA damage response, and differentiation (1-7). The SNF2H helicase facilitates DNA damage repair by actively moving nucleosomes for DNA damage response (DDR) proteins to effectively associate with damaged regions (3). Additional studies show that repair of double stranded breaks (DSBs) significantly decreases in the absence of SNF2H (3), and these cells become highly sensitive to DNA damage caused by x-rays and chemical treatments inducing DSBs (4,5).

Alternate Names

atp dependent chromatin remodeling

For Research Use Only. Not for Use in Diagnostic Procedures.
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