SOCS2 Antibody #2779
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
Activity of SOCS2 has been predominantly linked to growth hormone (GH) and insulin-like growth factor 1 (IGF-1) signaling but may also contribute to several biological processes including metabolism, bone formation, neuronal development, cancer, infection and other cytokine-dependent pathways (5). SOCS2 is widely expressed in adult and fetal tissues and is induced upon cytokine treatment (5,6). A number of studies suggest that SOCS2 can have either a positive or negative effect on GH/cytokine signaling (7-10). Mice deficient in SOCS2 grow signficantly larger than normal littermates (8). SOCS2 binds to tyrosine-phosphorylated GH and IGF-1 receptors via its SH2 domain, suppressing their signaling (6,9). In addition, the SOCS box of SOCS2 binds to Elongin B and C leading to activity as a ubiquitin ligase, promoting the degradation of the receptors as well as other SOCS family members (11-13).
- Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
- Chen, X.P. et al. (2000) Immunity 13, 287-90.
- Hilton, D.J. et al. (1998) Proc Natl Acad Sci USA 95, 114-9.
- Rico-Bautista, E. et al. (2006) Cytokine Growth Factor Rev 17, 431-9.
- Starr, R. et al. (1997) Nature 387, 917-21.
- Dey, B.R. et al. (1998) J Biol Chem 273, 24095-101.
- Favre, H. et al. (1999) FEBS Lett 453, 63-6.
- Metcalf, D. et al. (2000) Nature 405, 1069-73.
- Greenhalgh, C.J. et al. (2002) J Biol Chem 277, 40181-4.
- Greenhalgh, C.J. et al. (2005) J Clin Invest 115, 397-406.
- Tannahill, G.M. et al. (2005) Mol Cell Biol 25, 9115-26.
- Bullock, A.N. et al. (2006) Proc Natl Acad Sci U S A 103, 7637-42.
- Piessevaux, J. et al. (2006) J Biol Chem 281, 32953-66.
Limited Uses
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