Render Target: STATIC
Render Timestamp: 2024-11-22T11:46:32.577Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:59:42.250
Product last modified at: 2024-11-11T19:45:08.664Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SOD3 (E8M3G) Rabbit mAb #24000

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 28, 30
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SOD3 (E8M3G) Rabbit mAb recognizes endogenous levels of total SOD3 protein. This antibody cross-reacts with a band of unknown origin at approximately 18 kDa in some cell lines.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human SOD3 protein.

    Background

    Superoxide dismutase 3 (SOD3, EC-SOD) is a copper-containing antioxidant enzyme that plays a critical role in redox homeostasis in extracellular spaces (1). SOD3 localizes to the extracellular matrix through its C-terminal heparin binding domain, which interacts with proteoglycan on the cell surface (2). The enzyme catalyzes the dismutation of the superoxide radical (O2-) to hydrogen peroxide in the extracellular space, prevents oxidative fragmentation damage of the extracellular matrix under stress conditions (3), and protects against oxidant-mediated endothelial dysfunction and tissue inflammation (4,5). SOD3 antioxidative activity preserves the bioavailability of nitric oxide (NO), providing permissive signals through the normalization of tumor vasculature, leading to enhanced tumor infiltration by effector T cells (6,7). Downregulation of SOD3 provides a selective advantage for multiple cancer types (8). A SOD3 knockout animal model displays resistance to chemotherapy response, while overexpression of SOD3 increases the tumor response to chemotherapy (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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