SP1 (D4C3) Rabbit mAb #9389
- WB
- IP
- IHC
- F
- ChIP
- C&R
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:1000 - 1:4000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
| Chromatin IP | 1:100 |
| Chromatin IP-seq | 1:100 |
| CUT&RUN | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #84386.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Kadonaga, J.T. et al. (1987) Cell 51, 1079-90.
- Song, J. et al. (2003) Int J Mol Med 11, 547-53.
- Tan, N.Y. and Khachigian, L.M. (2009) Mol Cell Biol 29, 2483-8.
- Majumdar, G. et al. (2003) Am J Physiol Endocrinol Metab 285, E584-91.
- Majumdar, G. et al. (2006) J Biol Chem 281, 3642-50.
- Solomon, S.S. et al. (2008) Life Sci 83, 305-12.
- Citron, B.A. et al. (2008) J Neurosci Res 86, 2499-504.
Limited Uses
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