Render Target: STATIC
Render Timestamp: 2024-10-16T10:38:32.701Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-25 16:36:57.622
Product last modified at: 2024-09-28T08:00:10.963Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Spermine synthase (F9W1S) Rabbit mAb #96610

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 41
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Spermine synthase (F9W1S) Rabbit mAb recognizes endogenous levels of total spermine synthase protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human spermine synthase protein.

    Background

    Polyamines, including spermine, spermidine, and putrescine, are involved in many cellular processes, for example, cell proliferation, differentiation, apoptosis, the synthesis of proteins and nucleic acids, the regulation of ion channel activity, and the protection from oxidative injury (1). The cellular content of polyamines is tightly regulated; their biosynthesis is catalyzed by different enzymes, including spermine synthase (SMS) and spermidine synthase (SRM). The enzymes N1-acetylpolyamine oxidase (PAOX), spermidine/spermine N1-acetyltransferase (SAT1), and spermine oxidase (SMOX) are involved in the polyamine catabolism (1). SMS catalyzes spermine production from spermidine and decarboxylated S-adenosylmethionine (2). Mutations in SMS cause Snyder-Robinson Syndrome, characterized by developmental delay, muscle/bone abnormalities, and intellectual disability (3-6). A recent study has indicated the potential role of polyamines in autophagy and tauopathy, where a reduction in SMS had a protective effect against tauopathy in a Drosophila model and human cells (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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