Render Target: STATIC
Render Timestamp: 2024-11-21T13:57:08.840Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 02:00:01.334
Product last modified at: 2024-09-30T08:02:06.697Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Spi-C (E7V7F) Rabbit mAb #41910

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY Transfected Only
    MW (kDa) 25, 28
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Spi-C (E7V7F) Rabbit mAb recognizes overexpressed levels of total Spi-C protein. This antibody detects a 70 kDa band of unknown origin in some cell lines.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala205 of mouse Spi-C protein.

    Background

    Spi-C belongs to the erythroblast transformation-specific (ETS) domain family of transcription factors and binds to the purine-rich PU-box (1-3). Spi-C is a lineage-instructive factor involved in generating mature, specialized subsets of both myeloid and lymphoid cells (6). Spi-C is highly expressed by red pulp macrophages in the spleen, which are required for red blood cell recycling and iron homeostasis (4,6). Spi-C is also transiently expressed in B cells during specific stages of their development and regulates cell fate decisions at the pro-B to pre-B cell stage and plasma cell differentiation (1-3,6). Spi-C has also been shown to be a positive regulator that promotes osteoclast differentiation and function (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.