Spry2 (D3G1A) Rabbit mAb #14954
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Studies have shown that several cancers have reduced levels of Spry2 expression implicating Spry2 as a tumor suppressor (5-8). The regulation of Spry2 expression and activity appears to be a complex process involving casein kinase 1, Shp2 phosphatase, and Spry2-interacting partners (9-11). Phosphorylation of Tyr55 residue of Spry2 is required for the inhibitory function of Spry2 in FGF/MAPK signaling (12,13).
- Guy, G.R. et al. (2003) J Cell Sci 116, 3061-8.
- Guy, G.R. et al. (2009) J Endocrinol 203, 191-202.
- Lim, J. et al. (2000) J Biol Chem 275, 32837-45.
- Lim, J. et al. (2002) Mol Cell Biol 22, 7953-66.
- Herold, T. et al. (2014) Blood 124, 1304-11.
- Gao, M. et al. (2012) EMBO Mol Med 4, 776-90.
- Sánchez, A. et al. (2008) Oncogene 27, 4969-72.
- Frank, M.J. et al. (2009) Blood 113, 2478-87.
- Yim, D.G. et al. (2015) Oncogene 34, 474-84.
- Okur, M.N. et al. (2014) Mol Cell Biol 34, 271-9.
- Mason, J.M. et al. (2004) Mol Biol Cell 15, 2176-88.
- Edwin, F. et al. (2009) Mol Pharmacol 76, 679-91.
- Fong, C.W. et al. (2003) J Biol Chem 278, 33456-64.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.