SPT4 (D3P2W) Rabbit mAb #64828
- WB
- IP
- ChIP
- C&R
- C&T
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 13 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
- C&T-CUT & Tag
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:100 |
| CUT&RUN | 1:50 |
| CUT&Tag | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
- Wada, T. et al. (1998) Genes Dev 12, 343-56.
- Saunders, A. et al. (2006) Nat Rev Mol Cell Biol 7, 557-67.
- Gomes, N.P. et al. (2006) Genes Dev 20, 601-12.
- Yamaguchi, Y. et al. (1999) Cell 97, 41-51.
- Buratowski, S. (2009) Mol Cell 36, 541-6.
- Wada, T. et al. (1998) EMBO J 17, 7395-403.
- Yamada, T. et al. (2006) Mol Cell 21, 227-37.
Limited Uses
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