Render Target: STATIC
Render Timestamp: 2025-01-03T12:18:04.622Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-09-30 01:54:26.303
Product last modified at: 2025-01-01T09:05:50.639Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SPT6 (D6J9H) Rabbit mAb #15616

Filter:
  • WB
  • IP
  • ChIP
  • C&R
  • C&T

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 210
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    • C&R-CUT & RUN 
    • C&T-CUT & Tag 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

    The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.


    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Chromatin IP 1:50
    CUT&RUN 1:50
    CUT&Tag 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SPT6 (D6J9H) Rabbit mAb recognizes endogenous levels of total SPT6 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val112 of human SPT6 protein.

    Background

    SPT6 or SUPT6H is a histone H3 chaperone protein involved in transcriptional elongation and chromatin structure (1). The SPT6 protein contains a highly acidic N-terminus with leucine zipper and SH2 domains, which can interact with phospho-Rpb1 CTD (Ser2) to recruit SPN1 and other mRNA processing and export factors (2). SPT6 can enhance the elongation rate of RNA polymerase II, and can also maintain the modification state of histone H3 tails. (3-4). Loss of SPT6 causes improper initiation of transcription within coding regions (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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