Render Target: STATIC
Render Timestamp: 2024-12-20T11:39:29.006Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-01 12:01:06.313
Product last modified at: 2024-10-02T07:00:57.311Z
Cell Signaling Technology Logo
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. SPTLC1 (F7Y7S) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SPTLC1 (F7Y7S) Rabbit mAb #76977

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 50
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SPTLC1 (F7Y7S) Rabbit mAb recognizes endogenous levels of total SPTLC1 protein. This antibody does not cross-react with SPTLC2 or SPTLC3 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys207 of human SPTLC1 protein.

    Background

    The serine palmitoyltransferase (SPT) complex catalyzes the initial and rate-limiting step of de novo sphingolipid biosynthesis. This protein complex consists of an SPTLC1/SPTLC2 heterodimer or an SPTLC1/SPTLC3 heterodimer as the catalytic core (1-3). Studies show that SPTLC1 mutations leading to unregulated SPT activity and increased sphingolipid synthesis cause amyotrophic lateral sclerosis (ALS), a progressive neurodegenerative disease (4). In addition, mutations in SPTLC1 that change the substrate of SPT from serine to alanine drive the accumulation of deoxysphingolipids and cause neuropathy. Furthermore, dietary restriction of serine and glycine also leads to the accumulation of deoxysphingolipids in tumors and reduces tumor growth in mouse xenograft models (5).

    Database Links

    UniProt ID: O15269


    Entrez-Gene Id: 10558


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.