Render Target: STATIC
Render Timestamp: 2024-12-26T11:55:50.982Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:28.369
Product last modified at: 2024-12-11T13:00:13.637Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SRCAP (E9V8O) Rabbit mAb #28716

Filter:
  • WB
  • IP
  • ChIP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 400
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SRCAP (E9V8O) Rabbit mAb recognizes endogenous levels of total SRCAP protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro911 of human SRCAP protein.

    Background

    Snf2-Related CBP Activator Protein (SRCAP) is an ATPase that exists in a large complex that activates transcription by enhancing CBP activity and by depositing H2A.Z into nucleosomes (1-3). SRCAP can enhance nuclear receptor function along with CARM-1 and GRIP-1 (4). In prostate cancer, SRCAP associates with androgen receptor to control prostate specific antigen expression and increase cell growth (5). During double-stranded break repair, SRCAP is required for DNA-end resection and RAD51 recruitment (6). Mutations in SRCAP result in a developmental disorder called Floating-Harbor syndrome (7,8). SRCAP also plays a role in viral infection as it has been shown to interact with various viral proteins (9,10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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