SRF (D71A9) XP® Rabbit mAb #5147
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk Pg |
| SENSITIVITY | Endogenous |
| MW (kDa) | 67 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Pg-Pig
Product Information
Product Usage Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Treisman, R. (1995) EMBO J 14, 4905-13.
- Janknecht, R. et al. (1995) Carcinogenesis 16, 443-50.
- Marais, R.M. et al. (1992) EMBO J 11, 97-105.
- Rivera, V.M. et al. (1993) Mol Cell Biol 13, 6260-73.
- Miranti, C.K. et al. (1995) Mol Cell Biol 15, 3672-84.
- Heidenreich, O. et al. (1999) J Biol Chem 274, 14434-43.
Limited Uses
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