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Render Timestamp: 2024-11-22T11:08:12.151Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:36.049
Product last modified at: 2024-10-30T18:15:08.561Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. SRPK1 (E7J5U) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SRPK1 (E7J5U) Rabbit mAb #52012

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SRPK1 (E7J5U) Rabbit mAb recognizes endogenous levels of total SRPK1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys377 of human SRPK1 protein.

    Background

    Serine/arginine-rich protein-specific kinases (SRPKs) constitute a small family of serine/threonine protein kinases that phosphorylate serine residues in sequence regions containing repetitive serine/arginine-rich dipeptides (SR domains) (1). There are three known family members (SRPK1-3), of which SRPK1 and SRPK2 are the most well-studied. Multiple research studies have shown these kinases play important roles in post-transcriptional regulation, notably via phosphorylation of SR-family RNA splicing factors (2). Early studies of SRPK proteins in mice suggested that SRPK1 was expressed ubiquitously, whereas SRPK2 exhibited more tissue-specific expression patterns, while demonstrating conserved substrate specificity (3). SRPK2 has since been implicated in lipid biogenesis, following activation through sequential phosphorylation by mTORC-activated S6K1 and casein kinase 1 (5). Phosphorylation of SRPK2 at Ser494 and Ser497 promotes nuclear translocation of SRPK2, thereby enabling its regulation of mRNA splicing factors involved in lipid biogenesis. Notably, SRPK1 and SRPK2 have both been implicated in the phosphorylation of key viral proteins (5), including the nucleocapsid protein of the SARS and SARS-CoV-2 coronaviruses (6), a modification that may be essential for viral replication.

    Database Links

    UniProt ID: Q96SB4


    Entrez-Gene Id: 6732


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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