ST14/Matriptase Antibody #19271
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80, 95 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
ST14/Matriptase Antibody recognizes endogenous levels of total ST14/Matriptase protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn280 of human ST14/Matriptase protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Suppressor of tumorigenicity 14 protein (ST14), also known as matriptase, is a type II transmembrane protease (1,2). It is highly expressed in epithelia cells and important for the differentiation and homeostasis of epithelium (3). ST14 is synthesized as a single-chain precursor, and cleaved first at Gly149 in endoplasmic reticulum or Golgi. The resulting N-terminal and C-terminal fragments are non-covalently associated, and interact with hepatocyte growth factor activator inhibitor 1 (HAI-1), which facilitates the transportation of ST14 to the plasma membrane. Cell surface ST14 can be activated by autocleavage at Arg614 though an incompletely understood mechanism. HAI-1 inhibits activated ST14, and HAI-1 and ST14 complex can be shed from cell surface (4,5). In different context, ST14 has been reported either as a tumor suppressor or as a tumor promoter (6-8)
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