Render Target: STATIC
Render Timestamp: 2024-11-21T13:22:49.726Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-04-05 20:25:15.802
Product last modified at: 2024-10-28T08:30:09.917Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

STAM2 Antibody #14674

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 70
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    STAM2 Antibody recognizes endogenous levels of total STAM2 protein. This antibody does not cross-react with STAM1.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human STAM2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Signal transducing adaptor molecule 2 (STAM2) is a ubiquitously expressed STAM family adaptor protein and an integral component of the ESCRT-0 complex. Similar to STAM1, STAM2 possesses a single SH3 domain and an immunoreceptor tyrosine-based activation motif (ITAM). Following activation of multiple growth factor and cytokine cell surface receptors, the STAM2 protein undergoes tyrosine phosphorylation and potentiates mitogenic signals driven by these receptors (1,2). Research studies demonstrate that STAM2 is localized to complexes containing Eps15, Hrs, and STAM1 proteins on early endosome membranes. A tandem, amino-terminal VHS (Vps27/Hrs/STAM) domain and UIM (ubiquitin-interacting) motif within STAM2 facilitate STAM2 interaction with ubiquitinated cargo proteins, suggesting that this adaptor participates in the endosomal sorting of ubiquitinated proteins targeted for lysosomal degradation (3-6). Gene targeting studies have revealed an indispensible role for STAM2 in T-cell development (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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