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STEEP Antibody

STEEP Antibody #35136

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	28
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

STEEP Antibody recognizes endogenous levels of total STEEP protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human STEEP protein. Antibodies are purified by peptide affinity chromatography.

Background

STING ER exit protein (STEEP) is a highly conserved, ubiquitously expressed protein, which has been shown to be a positive regulator of the cGAS-STING signaling pathway. Mechanistically, research studies have demonstrated that STEEP constitutively interacts with STING to coordinate STING ER-to-Golgi trafficking, which is an essential step to ensure productive STING-dependent signaling and gene expression in response to viral infection (1).

Zhang, B.C. et al. (2020) Nat Immunol 21, 868-879.

Database Links

UniProt ID: Q9H5V9

Entrez-Gene Id: 63932

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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