Render Target: STATIC
Render Timestamp: 2024-12-20T11:15:18.048Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:56:58.350
Product last modified at: 2024-12-17T19:03:55.981Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. STEP (D9H3) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

STEP (D9H3) Rabbit mAb #9069

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 46, 61
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    STEP (D9H3) Rabbit mAb detects endogenous levels of total STEP46 and STEP61 proteins.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile440 of human STEP61 protein.

    Background

    Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer's disease (1).

    Database Links

    UniProt ID: P54829


    Entrez-Gene Id: 84867


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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