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Render Timestamp: 2024-12-30T10:56:09.768Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-19 13:11:06.267
Product last modified at: 2024-11-20T08:01:03.657Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. STIL (E4U6R) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

STIL (E4U6R) Rabbit mAb #38589

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  • WB

Inquiry Info. # 38589

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    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 170
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    STIL (E4U6R) Rabbit mAb recognizes endogenous levels of total STIL protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human STIL protein.

    Background

    STIL (SCL/TAL-1 interrupting locus) is an integral component of the complex required for centriole duplication during cell division (1). Centrioles are microtubule structures within the centrosome that have a critical role in the assembly of the mitotic spindle and templating the formation of primary cilia. Centriole duplication taking place during cell division involves the transient association of STIL with PLK4 and SAS-6 into a cartwheel structure (2). STIL directly interacts and is phosphorylated by PLK4 (3-7). Deletion of STIL in mice leads to lethality with a loss of centrioles and primary cilia (8). Mutation of the STIL gene in humans leads to neurological defects, including microcephaly (9-11). Expression of STIL has been associated with several cancer types. The STIL locus was originally identified in chromosomal aberration in T-cell leukemias (12). Elevated expression of STIL is observed in several cancer types, where it may contribute to increased mitotic activity, oncogenic signaling pathways, metastatic potential, and poor prognosis (13-18).
    1. Arquint, C. and Nigg, E.A. (2016) Biochem Soc Trans 44, 1253-1263.
    2. Gönczy, P. and Hatzopoulos, G.N. (2019) J Cell Sci 132, jcs228833. doi: 10.1242/jcs.228833.
    3. Arquint, C. et al. (2015) Elife 4, e07888. doi: 10.7554/eLife.07888.
    4. Ohta, M. et al. (2014) Nat Commun 5, 5267.
    5. Moyer, T.C. et al. (2015) J Cell Biol 209, 863-78.
    6. Kratz, A.S. et al. (2015) Biol Open 4, 370-7.
    7. Dzhindzhev, N.S. et al. (2014) Curr Biol 24, 2526-32.
    8. David, A. et al. (2014) Cell Cycle 13, 2859-68.
    9. Kakar, N. et al. (2015) Hum Genet 134, 45-51.
    10. Kumar, A. et al. (2009) Am J Hum Genet 84, 286-90.
    11. Bennett, H. et al. (2014) Pediatr Neurol 51, 434-6.
    12. Aplan, P.D. et al. (1992) Blood 79, 1327-33.
    13. Ramaswamy, S. et al. (2003) Nat Genet 33, 49-54.
    14. Erez, A. et al. (2004) Oncogene 23, 5371-7.
    15. Rabinowicz, N. et al. (2017) Oncotarget 8, 27380-27392.
    16. Yu, H. et al. (2022) Cancers (Basel) 14, 5777. doi: 10.3390/cancers14235777.
    17. Wu, X. et al. (2019) Gene 686, 220-227.
    18. Wang, J. et al. (2019) J Cell Mol Med 23, 5566-5575.

    Database Links

    UniProt ID: Q15468


    Entrez-Gene Id: 6491


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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