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Product last modified at: 2025-03-13T12:00:38.995Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Survivin (6E4) Mouse mAb #2802

Filter:
  • WB
Western Blotting Image 1: Survivin (6E4) Mouse mAb
Western blot analysis of extracts from 293 and HeLa cells, using Survivin (6E4) Mouse mAb.

To Purchase # 2802

Cat. # Size Qty. Price
2802T 20 µl
$152
2802S 100 µl
$336

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 16
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #83711.

Protocol

Specificity / Sensitivity

Survivin (6E4) Mouse mAb detects endogenous levels of human survivin. The antibody does not cross-react with other types of apoptotic inhibitor proteins.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human survivin.

Background

Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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