SV40 Large T Antigen (D1E9E) Rabbit mAb #15729

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Supporting Data

REACTIVITY	Vir
SENSITIVITY	Endogenous
MW (kDa)	90
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IF-Immunofluorescence

Species Cross-Reactivity Key:

Vir-Virus

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunofluorescence (Immunocytochemistry)	1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SV40 Large T Antigen (D1E9E) Rabbit mAb recognizes transgenic levels of total SV40 Large T Antigen protein.

Species Reactivity:

Virus

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys315 of SV40 Large T Antigen protein.

Background

Simian Virus 40 (SV40) is a small double-stranded, circular DNA tumor virus encoding three tumor antigens: large T, Small T, and 17KT. These antigens contribute to cellular transformation, an area of research where they are utilized extensively. Transformation of cells by SV40 large T antigen is mediated by its binding to the tumor suppressor proteins RB and p53 in the nucleus. Additional binding partners include p300, CBP, Cul7, IRS1, Bub1, Nbs1, and Fbw7, which also contribute to transformation (1).

Cheng, J. et al. (2009) Semin Cancer Biol 19, 218-28.

Database Links

UniProt ID: P03070

Entrez-Gene Id: 29031019

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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