Cell Signaling Technology Logo - Extra Large

Symplekin Antibody #9981

Filter:
  • WB
  • IP

Inquiry Info. # 9981

Please see our recommended alternatives.

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 141
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Symplekin Antibody recognizes endogenous levels of total symplekin protein. This antibody also cross-reacts with 40 and 55 kDa proteins of unknown origin in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp210 of human symplekin protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Symplekin is necessary for 3’-end cleavage and polyadenylation of mRNAs, histone 3’-end processing and polyadenylation in the cytoplasm (1-5). It is thought to act as a scaffolding protein that brings together factors involved in mRNA 3’-end processing (1,2). Symplekin also plays a role in transcription initiation and termination by RNA polymerase II (RNAPII) through bridging the interaction between the polyadenylation machinery and RNAPII (3,6,7). In addition to its role in mRNA 3’-end processing, research studies have shown that symplekin localizes at epithelial cell tight junctions where it may help to regulate tight junction assembly, thereby maintaining the integrity of the epithelial monolayer and cell polarity (8,9).

    Alternate Names

    FLJ27092; SPK; SYM; SYMPK; Symplekin

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.