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Product last modified at: 2025-01-01T09:01:54.918Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Synaptojanin-1 Antibody #80377

Filter:
  • WB
Western Blotting Image 1: Synaptojanin-1 Antibody
Western blot analysis of extracts from various tissues using Synaptojanin-1 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 80377

Cat. # Size Qty. Price Ships
80377S 100 µl
$306

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 173
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Synaptojanin-1 Antibody recognizes endogenous levels of total Synaptojanin-1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1186 of human Synaptojanin-1 protein. Antibodies are purified by peptide affinity chromatography.

Background

Synaptojanin-1 is a nerve terminal-enriched phosphatase that dephosphorylates phosphoinositides (1-3). Synaptojanin-1 contains an N-terminal Sac1 PI4P phosphatase domain (Sac domain) that dephosphorylates PI3P and PI(3,5)P2, a central 5-phosphatase domain that dephosphorylates PI(4,5)P2 and PI(3,4,5)P3, and an RNA recognition motif (4). In the brain, synaptic vesicles are released in response to action potentials, releasing neurotransmitters from synaptic vesicles and initiating interneuronal communication. As part of the synaptic vesicle cycle, fused synaptic vesicle membranes and proteins are endocytosed to be recycled to generate new synaptic vesicles in order to maintain neuronal function. A complex set of proteins, enriched in synaptic vesicles and synaptic membrane, via interaction with highly regulated lipid compositions at the synapse, dynamically regulate the synaptic vesicle cycling process (5). As part of that machinery, Synaptojanin-1 initiates completion of synaptic vesicle endocytosis by regulating, with its partner endophilin-A, the removal of clathrin and the formation of the vesicle “neck” (6). The dephosphorylation of PI(4,5)P2 at the synaptic membrane by Synaptojanin-1 phosphatase activity is critical in shedding endocytic factors from their membranes (4,7).  Recently, several genetic studies have linked proteins that participate in synaptic vesicle endocytic recycling, including Synaptojanin-1, to early onset Parkinson's disease (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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