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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Synaptophysin Antibody #4329

Filter:
  • WB
Western Blotting Image 1: Synaptophysin Antibody
Western blot analysis of extracts from rat brain, mouse brain and human cerebellum using Synaptophysin Antibody.

To Purchase # 4329

Cat. # Size Qty. Price
4329S 100 µl
$306

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 38
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Synaptophysin Antibody detects endogenous levels of total synaptophysin protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala230 of human synaptophysin. Antibodies are purified by peptide affinity chromatography.

Background

Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).
For Research Use Only. Not For Use In Diagnostic Procedures.
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