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Render Timestamp: 2024-12-20T11:25:20.577Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-08 22:05:24.266
Product last modified at: 2024-12-17T19:05:08.341Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Syndecan 1 (E7F7T) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Syndecan 1 (E7F7T) Rabbit mAb #96489

Filter:
  • IHC
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IHC-Immunohistochemistry 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:100 - 1:400
    Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200
    Flow Cytometry (Live) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #25851.

    Protocol

    Specificity / Sensitivity

    Syndecan 1 (E7F7T) Rabbit mAb recognizes endogenous levels of total syndecan 1 protein for approved applications. Non-specific staining was observed in skeletal muscle by immunohistochemistry.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus in the extracellular region of human syndecan 1 protein.

    Background

    Syndecans are a family of type 1 transmembrane heparan sulfate proteoglycans comprising four members in mammals (SDC1-4) (1) encoded by four syndecan genes. Syndecans are involved in embryonic development, tumorigenesis, and angiogenesis (2). The extracellular domain harbors attachment sites for heparan sulfate and chondroitin sulfate chains, facilitating interaction with an array of proteins, including a plethora of growth factors. In addition, the hydrophobic C-terminal intracellular domain can interact with proteins containing a PDZ domain (2). These interactions place syndecans as important integrators of membrane signaling (3). Syndecans undergo proteolytic cleavage causing the release of their extracellular domain (shedding), converting the membrane-bound proteins into soluble molecular effectors (4).

    Syndecan 1 (SDC1) is a specific marker for plasmacytic differentiation in hematologic disorders (5-7). This cell surface proteoglycan is also expressed in normal epithelial cells and tissues as well as various types of cancer tissues (8-11). The extracellular shed form of syndecan 1 remains soluble or accumulates in the extracellular matrix where it binds growth factors, cytokines and other extracellular matrix proteins (12,13). This binding activates signaling of bound growth factors or cytokines, which results in enhanced tumor growth, dissemination, angiogenesis, and osteolysis (14-17). As a result, the level of syndecan 1 protein and its shed form may serve as prognostic factors for a list of malignancies (6,18,19). Syndecan 1 has recently been found to be a critical mediator of macropinocytosis in pancreatic cancer (20).
    1. Couchman, J.R. (2003) Nat Rev Mol Cell Biol 4, 926-37.
    2. Multhaupt, H.A. et al. (2009) J Physiol Pharmacol 60 Suppl 4, 31-8.
    3. Zimmermann, P. and David, G. (1999) FASEB J 13 Suppl, S91-S100.
    4. Manon-Jensen, T. et al. (2010) FEBS J 277, 3876-89.
    5. Chilosi, M. et al. (1999) Mod Pathol 12, 1101-6.
    6. Seidel, C. et al. (2000) Blood 95, 388-92.
    7. O'Connell, F.P. et al. (2004) Am J Clin Pathol 121, 254-63.
    8. Inki, P. and Jalkanen, M. (1996) Ann Med 28, 63-7.
    9. Matsumoto, A. et al. (1997) Int J Cancer 74, 482-91.
    10. Conejo, J.R. et al. (2000) Int J Cancer 88, 12-20.
    11. Zellweger, T. et al. (2003) Prostate 55, 20-9.
    12. Bayer-Garner, I.B. et al. (2001) Mod Pathol 14, 1052-8.
    13. Ramani, V.C. et al. (2013) FEBS J 280, 2294-306.
    14. Derksen, P.W. et al. (2002) Blood 99, 1405-10.
    15. You, W.K. and McDonald, D.M. (2008) BMB Rep 41, 833-9.
    16. Ramani, V.C. et al. (2011) J Biol Chem 286, 6490-9.
    17. Aragão, A.Z. et al. (2012) PLoS One 7, e43521.
    18. Joensuu, H. et al. (2002) Cancer Res 62, 5210-7.
    19. Lee, S.H. et al. (2014) Int J Clin Oncol 19, 247-53.
    20. Yao, W. et al. (2019) Nature 568, 410-414.

    Database Links

    UniProt ID: P18827


    Entrez-Gene Id: 6382


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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