TAF1 (D6J8B) Rabbit mAb #12781
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 250 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
TBP-associated factor 1 (TAF1) is the largest subunit of TFIID and possesses multiple enzymatic activities including a protein kinase activity (4), a histone acetyltransferase activity (5), and both E1 and E2 ubiquitin-activating and conjugating activities (6,7). The target proteins of these enzymatic activities include both histones and non-histones and play a critical role in regulating transcription activation (3). In addition, TAF1 contains two tandem bromodomains that recognize acetylated histone H3 and histone H4 and are thought to promote the recruitment and association of TFIID at target promoters post activator recruitment of coactivators for gene expression (8,9).
- Goodrich, J.A. and Tjian, R. (1994) Curr Opin Cell Biol 6, 403-9.
- Berk, A.J. (2000) Cell 103, 5-8.
- Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78.
- Dikstein, R. et al. (1996) Cell 84, 781-90.
- Mizzen, C.A. et al. (1996) Cell 87, 1261-70.
- Pham, A.D. and Sauer, F. (2000) Science 289, 2357-60.
- Boutet, S.C. et al. (2010) Mol Cell 40, 749-61.
- Jacobson, R.H. et al. (2000) Science 288, 1422-5.
- Kanno, T. et al. (2004) Mol Cell 13, 33-43.
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