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Product last modified at: 2025-01-01T09:03:23.637Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Tau (GT-38) Mouse mAb (BSA and Azide Free) #59939

Filter:
  • IHC
  • ELISA
Immunohistochemistry Image 1: Tau (GT-38) Mouse mAb (BSA and Azide Free)
Immunohistochemical analysis of two cases of paraffin-embedded human Alzheimer's brain using Tau (GT-38) Mouse mAb (top) or Tau (D1M9X) XP® Rabbit mAb #46687 (bottom). Data were generated using the standard formulation of this product.

To Purchase # 59939

Cat. # Size Qty. Price Ships
59939SF 100 µg
$876

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG1 kappa
Application Key:
  • IHC-Immunohistochemistry 
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

This product is the carrier free version of product #66850. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #66850

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

Tau (GT-38) Mouse mAb (BSA and Azide Free) recognizes paired helical filament conformational tau protein. This antibody preferentially recognizes tau conformations related to Alzheimer's disease compared to other tauopathies.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with tau paired helical filaments from human Alzheimer's disease brain.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Alternative splicing of exon 10 results in the expression of two groups of tau: three-repeat and four-repeat tau. Isoforms 2, 4, and 5 express three microtubule binding repeat domains (Tau 3R) while isoforms 6, 7, and 8 express four microtubule binding repeat domains (Tau 4R) (4). Expression of Tau 3R and Tau 4R in cells can be different in mild or pathological conditions. For example, Tau 3R is preferentially expressed in Pick's disease (PiD) and corticobasal degeneration (CBD), while Tau 3R and Tau 4R are equally expressed in AD (5,6). The repeat-dependent tau has a different pattern of phosphorylation in different diseases, and also has the ability and patterns of aggregation (7-9). These varying patterns of aggregation result in disease specific conformational structures, including hyperphosphorylated straight filaments (SFs) and PHFs in AD compared to SFs and twisted filaments in both PiD and CBD (10,11).
For Research Use Only. Not For Use In Diagnostic Procedures.
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