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XML generation date: 2025-03-07 13:11:09.054
Product last modified at: 2025-02-07T20:15:09.294Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TBC1D1 (D2Y8M) Rabbit mAb #66433

Filter:
  • WB
  • IP
Western Blotting Image 1: TBC1D1 (D2Y8M) Rabbit mAb
Western blot analysis of extracts from C2C12 myoblasts, mouse tibialis anterior muscle and mIMCD3 cells using TBC1D1 (D2Y8M) Rabbit mAb.

To Purchase # 66433

Cat. # Size Qty. Price
66433T 20 µl
$153
66433S 100 µl
$339

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 160
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

TBC1D1 (D2Y8M) Rabbit mAb recognizes endogenous levels of total TBC1D1 protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val796 of mouse TBC1D1 protein.

Background

TBC1D1 is a paralog of AS160 (1) and both proteins share about 50% identity (2). TBC1D1 was shown to be a candidate gene for severe obesity (3). It plays a role in Glut4 translocation through its GAP activity (2,4). Studies indicate that TBC1D1 is highly expressed in skeletal muscle (1). Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue (1). Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified in skeletal muscle (5). Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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