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Render Timestamp: 2024-11-29T11:30:44.149Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-09-30 01:59:11.600
Product last modified at: 2024-09-30T08:01:53.954Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. TCF12/HEB (E8R5E) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TCF12/HEB (E8R5E) Rabbit mAb #95359

Filter:
  • WB
  • IP
  • IF
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 85
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600
    Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TCF12/HEB (E8R5E) Rabbit mAb recognizes endogenous levels of total TCF12/HEB protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp60 of human TCF12/HEB protein.

    Background

    TCF12/HEB (T cell factor 12/HeLa E-box binding protein) is a member of the E proteins that are type I basic helix-loop-helix domain-containing transcription factors that bind directly to the E-box DNA consensus sequence and control numerous developmental processes (1,2). E protein family members include E2A, E2-2, and TCF12/HEB. TCF12/HEB forms homo- or heterodimers with other E proteins to regulate transcription of target genes that play a critical role in the development of T cells, B cells, and plasmacytoid dendritic cells (2-4). The TCF12/HEB gene has two transcriptional start sites leading to the generation of the long form called HEBCan, and the short form, HEBAlt (5). The two isoforms are thought to have distinct functions, with HEBCan playing a role throughout T cell development, while HEBAlt functions in the efficient generation of T cell precursors (5).

    Database Links

    UniProt ID: Q99081


    Entrez-Gene Id: 6938


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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