TdTomato (E3G5L) Rabbit mAb #20163
- WB
- IHC
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
TdTomato is a fluorescent, photostable orange dye that was isolated from and further engineered from the Discosoma sea anemone (1). Reported to be six times brighter than EGFP, TdTomato is comprised of a tandem dimer of monomers engineered from DsRed; the dimers are held together by intramolecular bonds. For this reason, TdTomato is considered a monomer. Its peak fluorescent excitation is at 554 nm and emission is at 581 nm (1).
Limited Uses
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