Render Target: STATIC
Render Timestamp: 2025-01-23T17:39:17.108Z
Commit: 8d9f38232df81570bbc23eaa560b31cb39dd8776
XML generation date: 2024-08-01 15:27:47.453
Product last modified at: 2024-05-30T07:03:56.675Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

TEAD1 Antibody #8526

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Inquiry Info. # 8526

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    Supporting Data

    REACTIVITY H R Mk
    SENSITIVITY Endogenous
    MW (kDa) 50
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TEAD1 Antibody recognizes endogenous levels of total TEAD1 protein. This antibody does not cross-react with other TEAD proteins.

    Species Reactivity:

    Human, Rat, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TEAD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of Yes-associated protein (YAP), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). When the Hippo pathway is turned on, YAP is phosphorylated by LATS1/2 kinase and sequestered in the cytoplasm by 14-3-3 protein binding, rendering YAP inactive. When the Hippo pathway is off, non-phosphorylated YAP translocates to the nucleus where it associates with various transcription factors including members of the transcriptional enhancer factor (TEF) family, also known as the TEA domain (TEAD) family (TEAD1-4) (5,6). Although widely expressed in tissues, the TEAD family proteins have specific tissue and developmental distributions. YAP/TEAD complexes regulate the expression of genes involved in cell proliferation and apoptosis (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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