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Render Timestamp: 2024-11-22T11:06:45.923Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:57:51.201
Product last modified at: 2024-11-07T16:15:08.976Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Tenascin C (E5J3B) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Tenascin C (E5J3B) Rabbit mAb #33352

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 200, 240
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Tenascin C (E5J3B) Rabbit mAb recognizes endogenous levels of total Tenascin C protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Tenascin C protein.

    Background

    Tenascin C is a large hexameric extracellular matrix glycoprotein that exhibits de-adhesive effects on cell-matrix interaction, enhancing cell proliferation and motility in most cell types. It is highly expressed in remodeling tissues during embryonic development and under pathological conditions in adults, and research studies have shown markedly increased expression in cancerous tissues (1,2). Tenascin C has been implicated in a variety of cellular processes relevant to atherosclerosis, including cell proliferation, migration, and apoptosis. Expression of Tenascin C is tightly controlled in adults and is upregulated in tissues undergoing wound healing (3). In development, the expression of Tenascin C is known to be associated with epithelial-mesenchymal transition (EMT) events, including gastrulation and formation of the neural crest, endocardial cushion, and secondary palate (1). Investigators have shown that Tenascin C is a key determinant of the tumor stroma and is involved in the initiation of tumorigenesis and progression to metastasis (2). Immature and mature astrocytes, radial glial cells, Schwann cells, and a subset of neurons express Tenascin C. Upon CNS trauma or exposure of neurons to excitotoxic agents, Tenascin C expression is upregulated by glial cells. Research studies have shown that Tenascin C is involved in guidance of migrating axons and neurons, synaptic plasticity, and neuronal regeneration, promoting spinal cord regeneration after injury (4).

    Database Links

    UniProt ID: P24821


    Entrez-Gene Id: 3371


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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