TFEB (D2O7D) Rabbit mAb #37785
- WB
- IP
- IHC
- ChIP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65-70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:300 - 1:1200 |
| Chromatin IP | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #51406.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Sardiello, M. et al. (2009) Science 325, 473-7.
- Sardiello, M. and Ballabio, A. (2009) Cell Cycle 8, 4021-2.
- Settembre, C. et al. (2011) Science 332, 1429-33.
- David, R. (2011) Nat Rev Mol Cell Biol 12, 404.
- Cuervo, A.M. (2011) Science 332, 1392-3.
- Peña-Llopis, S. et al. (2011) EMBO J 30, 3242-58.
- Settembre, C. and Ballabio, A. (2011) Autophagy 7, 1379-81.
- Peña-Llopis, S. and Brugarolas, J. (2011) Cell Cycle 10, 3987-8.
- Settembre, C. et al. (2012) EMBO J 31, 1095-108.
- Martina, J.A. et al. (2012) Autophagy 8, 903-14.
Limited Uses
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