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Render Timestamp: 2024-11-21T14:03:32.366Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:59:23.681
Product last modified at: 2024-10-08T15:15:09.471Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. TFII-I (E9U2N) Rabbit mAb (ChIP Formulated)
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TFII-I (E9U2N) Rabbit mAb (ChIP Formulated) #87604

Filter:
  • ChIP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TFII-I (E9U2N) Rabbit mAb (ChIP Formulated) recognizes endogenous levels of total TFII-I protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val263 of human TFII-I protein.

    Background

    TFII-I (also known as SPIN and BAP-135) is a multifunctional transcription factor that facilitates basal transcriptional machinery assembly at the core promotor region, as well as the assembly of the transcriptional activator complex at upstream regulatory sites (1). Four isoforms of TFII-I (alpha, beta, gamma, and delta) form homo- or heteromeric complexes, which may perform different functions on different promoters (1). In B cells, cross-linking of BCR (B cell receptors) leads to TFII-I phosphorylation at Tyr248 by Btk (2). This phosphorylation disrupts the association of Btk and TFII-I and enhances TFII-I transcriptional activity and nuclear localization (2). In nonlymphoid cells, TFII-I is phosphorylated at Tyr248 by Src dependent kinase or JAK2 (3,4). PKG (cGMP-dependent kinase) interacts with and phosphorylates TFII-I at Ser371 and 743, which also promotes TFII-I transcription activity (5). TFII-I activity is also modulated by HDAC3 (Histone Deacetylase 3) through a physical interaction between the two proteins (6).

    Database Links

    UniProt ID: P78347


    Entrez-Gene Id: 2969


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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