Render Target: STATIC
Render Timestamp: 2024-11-21T13:12:59.466Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:55:20.545
Product last modified at: 2024-10-25T14:15:10.296Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

TFIIB (2F6A3H4) Mouse mAb #4169

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 32
    Source/Isotype Mouse IgG2b
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TFIIB (2F6A3H4) Mouse mAb detects endogenous levels of total TFIIB protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant human TFIIB protein.

    Background

    TFIIB (also known as GTF2B, TF2B and general transcription factor IIB) is a ubiquitously expressed transcription factor that plays a central role in the assembly of the transcription pre-initiation complex through direct recruitment of RNA polymerase II (1,2). TFIIB functions as a bridge between promoter-bound TFIID and RNA polymerase II. In addition to interacting with promoter-bound TFIID and TFIIA, TFIIB makes extensive contacts with the core promoter via two independent DNA-binding modules. TFIIB may also be a target of transcriptional activator proteins that act to stimulate pre-initiation complex assembly.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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