Render Target: STATIC
Render Timestamp: 2025-03-14T10:34:04.334Z
Commit: a619ae74f66dae0f27639e88da12bcf600e46428
XML generation date: 2025-03-07 13:11:35.363
Product last modified at: 2025-01-28T21:15:10.515Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

TFIIF-α Antibody #8080

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 74
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TFIIF-α Antibody recognizes endogenous levels of total TFIIF-α protein.

    Species Reactivity:

    Human, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Dog

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TFIIF-α protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    TFIIF is a member of the group of general transcription factors that facilitate the binding of RNA polymerase II (Pol II) to promoter sequences as part of the pre-initiation complex (PIC) (1). TFIIF consists of subunits TFIIF-α (RAP74) and TFIIF-β (RAP30). It is involved in the stabilization of Pol II association with the PIC and selection of the transcription start site during transcription initiation (1,2). In addition to its role in transcription initiation, TFIIF has been shown to stimulate the transcription elongation activity of Pol II as well as dephosphorylation and recycling of Pol II during transcription termination (3-5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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