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Inquiry Info. # 3712

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Supporting Data

REACTIVITY	H R Mi
SENSITIVITY	Endogenous
MW (kDa)	52
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
R-Rat
Mi-Mink

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

TGF-β Receptor I Antibody detects endogenous levels of total TGF-β receptor I protein independent of phosphorylation.

Species Reactivity:

Human, Rat, Mink

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central intracellular region of human TGF-β receptor I. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Transforming growth factor-β (TGF-β) proteins belong to the TGF-β superfamily of cytokines that play a critical role in regulating cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-3). TGF-β ligands elicit signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII) TGF-β receptors. Type I and type II receptors are serine/threonine kinases that form a heteromeric complex following ligand binding to the type II receptor. In response to ligand binding, the type II receptors form a stable complex with the type I receptors, triggering phosphorylation and activation of the type I receptor (4). This results in the recruitment of receptor-mediated SMADs (SMAD2, SMAD3), which are phosphorylated by the type I kinase in an SSXS domain in the C-terminus. This leads to recruitment of the co-SMAD (SMAD4), and subsequent translocation of this heteromeric SMAD complex to the nucleus, where it regulates transcription of target genes (5-7). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain. Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex (8).

Database Links

UniProt ID: P36897

Entrez-Gene Id: 7046

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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TGF-β Receptor I Antibody #3712