Render Target: STATIC
Render Timestamp: 2024-12-20T11:19:49.232Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:53:43.075
Product last modified at: 2024-12-17T18:47:56.140Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ThPOK (D9V5T) Rabbit mAb #13205

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ThPOK (D9V5T) Rabbit mAb recognizes endogenous levels of total ThPOK protein. This antibody cross-reacts with a 40 kDa protein of unknown origin.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant human ThPOK protein.

    Background

    The transcription factor Th-inducing POZ/Krüppel-like factor (ThPOK, ZBTB7B, cKROX, ZFP67) is a transcriptional repressor belonging to the POK/ZBTB family of lymphoid cell development regulators (1). ThPOK is best known as a signature CD4+ Th cell transcription factor that is upregulated during the differentiation of CD4+ Th but not CD8+ cytotoxic T cells (1). Expression of ThPOK in developing T cells represses expression of CD8 and cytotoxic T cell effector genes, and indirectly promotes expression of CD4 by antagonizing RUNX-mediated CD4 repression (2-4). ThPOK expression has also been observed in NKT cells and γδ T cells (5,6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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