Render Target: STATIC
Render Timestamp: 2025-01-28T12:09:48.627Z
Commit: 8d9f38232df81570bbc23eaa560b31cb39dd8776
XML generation date: 2024-09-20 06:19:08.775
Product last modified at: 2025-01-01T09:01:25.480Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Thymidine Kinase 1 Antibody #8960

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 26
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Thymidine Kinase 1 Antibody recognizes endogenous levels of total TK1 protein. This antibody does not cross-react with TK2 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly213 of human TK1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Thymidine kinases play a critical role in generating the DNA synthetic precursor deoxythymidine triphosphate (dTTP) by catalyzing the phosphotransfer of phosphate from ATP to deoxythymidine (dT) and thymidine (T) in the cell. There are two known thymidine kinases, cytoplasmic thymidine kinase 1 (TK1) and mitochondrial thymidine kinase 2 (TK2) (1,2). Unlike TK2, which is not modulated by the cell cycle, TK1 expression and activity is regulated in a cell cycle-dependent manner, accumulating during G1-phase to peak levels in S-phase before being degraded prior to cell division (3,4). Stability, but not activity, may be regulated via phosphorylation of TK1 at Ser13 by Cdc2 and/or Cdk2, but the precise mode of regulation remains elusive (5). These observations indicate that TK1 might be a useful marker of cell proliferation; however, recent studies have shown that TK1 plays a more significant role in the DNA damage response (6). Genotoxic stress promotes increased TK1 expression and kinase activity resulting in reduced cellular apoptosis and enhanced DNA repair efficiency (6). More importantly, numerous studies show that TK1 expression and activity are upregulated during neoplasia and disease progression in humans, and increased serum levels of TK1 correlate with poor prognosis and decreased survival in patients with various types of advanced tumors (7-12).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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