Thymidylate Synthase (D5B3) XP® Rabbit mAb #9045
- WB
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 30 |
| Source/Isotype | Rabbit |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 |
Storage
For a carrier free (BSA and azide free) version of this product see product #81327.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Levels of thymidylate synthase can be modultated in vitro by treatment with various drugs, such as 5-FU and lapatinib. 5-FU treatment induces the formation of two distinct bands, one at 35 kDa and another at 38 kDa, while lapatinib treatment results in a decrease in thymidylate synthase expression (11,12).
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- Aschele, C. et al. (2002) Ann Oncol 13, 1882-92.
- Jackman, A.L. and Calvert, A.H. (1995) Ann Oncol 6, 871-81.
- Van Triest, B. et al. (2000) J Histochem Cytochem 48, 755-60.
- Johnston, P.G. et al. (1994) J Clin Oncol 12, 2640-7.
- Kwon, H.C. et al. (2007) Ann Oncol 18, 504-9.
- Allegra, C.J. et al. (2002) J Clin Oncol 20, 1735-43.
- Allegra, C.J. et al. (2003) J Clin Oncol 21, 241-50.
- Tsourouflis, G. et al. (2008) Dig Dis Sci 53, 1289-96.
- Kim, S.H. et al. (2009) Am J Clin Oncol 32, 38-43.
- Chu, E. et al. (1993) Mol Pharmacol 43, 527-33.
- Kim, H.P. et al. (2009) PLoS One 4, e5933.
Limited Uses
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