Thyroid Transcription Factor 1 (TTF-1) (SPT24) Mouse mAb #23192
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| IHC Leica Bond | 1:100 - 1:400 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Thyroid Transcription Factor 1 (TTF-1) (SPT24) Mouse mAb recognizes endogenous levels of TTF-1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to a 123 amino acid fragment of the N-terminal region of human TTF-1 protein.
Background
Thyroid Transcription Factor 1 (TTF-1, also known as NKX2-1), a member of the NKX homeobox transcription factor family, was initially discovered in the FRTL-5 rat thyroid cell line (1). Subsequent studies have shown that TTF-1 plays an important role in differentiation and morphogenesis of the developing thyroid, lung, and ventral forebrain (2). TTF-1 controls the expression of several genes, some of which are tissue specific, such as: thyroglobulin, thyroperoxidase, and the thyrotropin receptor in the thyroid; and surfactant proteins and clara cell secretory protein in the lung (2,3). Investigators have found that TTF-1 is expressed in malignant tumors of the thyroid and lung, and it is commonly used as a marker for both primary and malignant lung cancers (4-6).
- Civitareale, D. et al. (1989) EMBO J 8, 2537-42.
- Boggaram, V. (2009) Clin Sci (Lond) 116, 27-35.
- Yamaguchi, T. et al. (2012) Cancer Cell 21, 348-61.
- Whithaus, K. et al. (2012) Arch Pathol Lab Med 136, 155-62.
- Yoshida, A. et al. (2011) Lung Cancer 72, 309-15.
- Moldvay, J. et al. (2004) Pathol Oncol Res 10, 85-8.
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