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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

TIAM1 Antibody #63647

Filter:
  • WB
Western Blotting Image 1: TIAM1 Antibody
Western blot analysis of extracts from various cell lines using TIAM1 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 63647

Cat. # Size Qty. Price Ships
63647S 100 µl
$306 Jan 22

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 220
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

TIAM1 Antibody recognizes endogenous levels of total TIAM1 protein.

Species Reactivity:

Human

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIAM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

TIAM1 (T-lymphoma invasion and metastasis-inducing protein 1) is a multidomain guanine nucleotide exchange factor (GEF) protein that activates Rac1, a GTPase involved in cytoskeletal dynamics that regulate cell migration, growth, and survival. TIAM1 also has been identified as an inhibitor of the YAP/TAZ signaling pathway, with two distinct subcellular mechanisms of action: (1) promoting cytoplasmic (proteosomal) degradation of YAP and TAZ; and (2) blocking the transcriptional co-activator functions of YAP and TAZ in the nucleus (3,4). The effects of TIAM1 on tumor development are also complex and context-dependent. For example, it has been reported that TIAM1 can promote tumor growth and progression in some contexts, while antagonizing tumor metastasis and invasion in other contexts (5,6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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