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XML generation date: 2024-09-30 01:57:58.745
Product last modified at: 2025-01-09T14:15:24.146Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TIAM1 (E5T7H) Rabbit mAb #31128

Filter:
  • WB
  • IP
Western Blotting Image 1: TIAM1 (E5T7H) Rabbit mAb
Western blot analysis of extracts from various cell lines using TIAM1 (E5T7H) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The absence of TIAM1 protein in HuH-7 cells is consistent with mRNA expression profiles reported in public bioinformatic databases, confirming specificity of the antibody for TIAM1.

To Purchase # 31128

Cat. # Size Qty. Price Ships
31128T 20 µl
$153
31128S 100 µl
$339

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 220
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

TIAM1 (E5T7H) Rabbit mAb recognizes endogenous levels of total TIAM1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg1585 of human TIAM1 protein.

Background

TIAM1 (T-lymphoma invasion and metastasis-inducing protein 1) is a multidomain guanine nucleotide exchange factor (GEF) protein that activates Rac1, a GTPase involved in cytoskeletal dynamics that regulate cell migration, growth, and survival. TIAM1 also has been identified as an inhibitor of the YAP/TAZ signaling pathway, with two distinct subcellular mechanisms of action: (1) promoting cytoplasmic (proteosomal) degradation of YAP and TAZ; and (2) blocking the transcriptional co-activator functions of YAP and TAZ in the nucleus (3,4). The effects of TIAM1 on tumor development are also complex and context-dependent. For example, it has been reported that TIAM1 can promote tumor growth and progression in some contexts, while antagonizing tumor metastasis and invasion in other contexts (5,6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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