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Render Timestamp: 2024-12-20T10:51:15.221Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:13.175
Product last modified at: 2024-12-17T19:01:23.031Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. TIF1α/TRIM24 (E9T3N) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TIF1α/TRIM24 (E9T3N) Rabbit mAb #79030

Filter:
  • WB
  • IP
  • IHC

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 140, 160, 180
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TIF1α/TRIM24 (E9T3N) Rabbit mAb recognizes endogenous levels of total TIF1α/TRIM24 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human TIF1α/TRIM24 protein.

    Background

    TIF1α, also known as TRIM24, is a member of the TIF1 (transcriptional intermediary factor 1) family, a group of transcriptional regulators that play key roles in development and differentiation. Members of this family are characterized by the presence of two conserved motifs – an N-terminal RING-B box-coiled-coil motif and a C-terminal PHD finger and bromodomain unit (1,2). TIF1α binds to the ligand binding domain of several nuclear receptors to regulate their transcriptional activity (2-4). While initially found to attenuate activities of RAR and protect against hepatocellular carcinomas, TIF1α also plays a role in ERα, AR, and VDR signaling (5-9). TIF1α also functions as an E3 ubiquitin ligase, and negatively regulates p53 (10,11). TIF1α is phosphorylated by ATM at Ser768 upon DNA damage, a modification which disrupts TIF1α-p53 interaction (12). TIF1α is overexpressed in numerous cancer types, and acts via numerous oncogenic signaling pathways including PI3K/Akt, Wnt, and AMPK (13-17).
    1. Le Douarin, B. et al. (1995) EMBO J 14, 2020-33.
    2. Le Douarin, B. et al. (1996) EMBO J 15, 6701-15.
    3. Heery, D.M. et al. (1997) Nature 387, 733-6.
    4. Le Douarin, B. et al. (1998) J Steroid Biochem Mol Biol 65, 43-50.
    5. Khetchoumian, K. et al. (2008) Cell Cycle 7, 3647-52.
    6. Ignat, M. et al. (2008) Proc Natl Acad Sci U S A 105, 2598-603.
    7. Khetchoumian, K. et al. (2007) Nat Genet 39, 1500-6.
    8. Tsai, W.W. et al. (2010) Nature 468, 927-32.
    9. Kikuchi, M. et al. (2009) Biochim Biophys Acta 1793, 1828-36.
    10. Allton, K. et al. (2009) Proc Natl Acad Sci U S A 106, 11612-6.
    11. Tai, E. and Benchimol, S. (2009) Proc Natl Acad Sci U S A 106, 11431-2.
    12. Jain, A.K. et al. (2014) Mol Cell Biol 34, 2695-709.
    13. Li, H. et al. (2012) PLoS One 7, e37657.
    14. Zhang, L.H. et al. (2015) Oncogene 34, 600-10.
    15. Fang, Z. et al. (2017) Oncol Lett 13, 1797-1806.
    16. Lv, D. et al. (2017) Nat Commun 8, 1454.
    17. Zhu, Y. et al. (2018) Exp Cell Res 367, 274-281.

    Database Links

    UniProt ID: O15164


    Entrez-Gene Id: 8805


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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