Render Target: STATIC
Render Timestamp: 2024-11-22T11:56:21.386Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-04-05 20:46:23.075
Product last modified at: 2024-11-11T14:00:18.420Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

TMEM41B Antibody #68071

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 22
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TMEM41B Antibody recognizes endogenous levels of total TMEM41B protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TMEM41B protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    TMEM41B (also known as Stasimon) was discovered as a target of splicing dysfunction induced by loss of spinal motor neuron (SMN) protein, a feature of spinal muscular atrophy (SMA), resulting in motor circuit defects (1). Loss of TMEM41B in mice results in embryonic lethality (2). TMEM41B is a transmembrane protein that resides at the endoplasmic reticulum (ER) and is found at contact sites between the ER and mitochondria (2). Independent CRISPR screens identified TMEM41B as a novel regulator of autophagy, being structurally and functionally related to VMP1 (3-5). Loss of TMEM41B inhibited autophagosome formation and autophagic flux (3-5). Furthermore, depletion of TMEM41B led to an accumulation of lipid droplets, indicating a role in lipid mobilization and utilization of fatty acids (3,4). TMEM41B also appeared in screens, identifying it as an essential host protein for infection of flaviviruses and coronaviruses, including SARS-CoV-2 (6,7).
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