TopBP1 (D8G4L) Rabbit mAb #14342
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 170 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
TopBP1 also regulates initiation of DNA replication along with the DNA replication factor treslin (6,7). TopBP1 has been shown to prevent replication associated DNA damage during neurogenesis (8), and to interact with mutant p53, mediating mutant p53 gain-of-function activity such as growth promotion and resistance to chemotherapeutic drugs (9). Phosphorylation of TopBP1 at Ser1159 by Akt regulates TopBP1 oligomerization and function in E2F1-dependent transcriptional regulation (10).
- Garcia, V. et al. (2005) DNA Repair (Amst) 4, 1227-39.
- Lindsey-Boltz, L.A. and Sancar, A. (2011) J Biol Chem 286, 19229-36.
- Burrows, A.E. and Elledge, S.J. (2008) Genes Dev 22, 1416-21.
- Duursma, A.M. et al. (2013) Mol Cell 50, 116-22.
- Wang, J. et al. (2013) Mol Cell 52, 667-78.
- Kumagai, A. et al. (2010) Cell 140, 349-59.
- Boos, D. et al. (2013) Science 340, 981-4.
- Lee, Y. et al. (2012) Nat Neurosci 15, 819-26.
- Liu, K. et al. (2011) Mol Cell Biol 31, 4464-81.
- Liu, K. et al. (2013) Mol Cell Biol 33, 4685-700.
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