TPI1 (F6U6Y) Rabbit mAb #34088

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	25
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:50 - 1:250

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

TPI1 (F6U6Y) Rabbit mAb recognizes endogenous levels of total TPI1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly234 of human TPI1 protein.

Background

Triosephosphate isomerase (TPI1) catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GAP). TPI1 expression levels were shown to increase in lung adenocarcinomas and inversely correlate with overall patient survival (1). In addition, mTORC1 phosphorylates and activates cyclin-dependent kinase 2 (CDK2). Activated CDK2, in turn, phosphorylates TPI1 at Ser117, stimulating TPI1 to translocate to the nucleus. Nuclear TPI1 reduces DHAP levels, leading to acetate accumulation in the nucleus and, therefore, impacting histone acetylation (2). Furthermore, the salt-inducible kinases (SIKs), which are activated by the serine/threonine protein kinase LKB1, phosphorylate TPI1 at Ser21 to regulate its activity in human lung adenocarcinomas (3).

Database Links

UniProt ID: P60174

Entrez-Gene Id: 7167

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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