Render Target: STATIC
Render Timestamp: 2025-01-03T11:43:06.922Z
Commit: 286c369131ceeedcf44c821941824d8d7e009e57
XML generation date: 2024-09-30 01:59:27.987
Product last modified at: 2025-01-01T09:07:16.934Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TPPP/p25 (E9C8G) Rabbit mAb #17843

Filter:
  • IF

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Immunofluorescence (Frozen) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TPPP/p25 (E9C8G) Rabbit mAb recognizes endogenous levels of total TPPP/p25 protein. Nonspecific labeling of the epithelium of fixed frozen mouse colon has been observed by immunofluorescence.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse TPPP/p25 protein.

    Background

    Tubulin polymerization-promoting protein (TPPP, also known as p25) is a microtubule nucleation factor in oligodendrocytes. As a largely unstructured protein, TPPP/p25 induces microtubule polymerization in vitro (1,2). TPPP/p25 is enriched in oligodendrocytes in the brain and may play a unique role in the structure and function of these unique cells (3,4). Oligodendrocytes extend elaborate microtubule arbors that contact neuronal axon segments to generate a myelin sheath that is critical for proper propagation of axonal action potentials. TPPP/p25 may not only play a critical role in establishing the microtubule network for oligodendrocytes, but may also contribute to the generation of Golgi outposts, which are Golgi found outside of the cell body. Golgi outposts may play a specialized role in building the local cytoarchitecture of oligodendrocytes and other brain cells (5,6). Misregulation of TPPP/p25 is linked to several neurodegenerative diseases, including Parkinson’s disease, and may contribute to disease etiology and/or represent a potential therapeutic target (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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