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XML generation date: 2024-09-20 06:21:33.177
Product last modified at: 2024-09-27T20:30:09.711Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. TRAF Antibody Sampler Kit

TRAF Antibody Sampler Kit #8347

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The TRAF Antibody Sampler Kit provides an economical means to evaluate endogenous levels of TRAF1, 2, 3, and 6. The kit contains enough primary and secondary antibodies to perform two western mini-blot experiments.

    Specificity / Sensitivity

    TRAF1 (45D3) Rabbit mAb detects endogenous levels of total TRAF1 protein. TRAF2 (C192) Antibody detects endogenous levels of total human TRAF2 protein. TRAF3 Antibody detects endogenous levels of total TRAF3 protein. TRAF6 (D21G3) Rabbit mAb detects endogenous levels of total TRAF6 protein. Cross-reactivity was not detected with other family members at physiological conditions.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys57 of human TRAF1 or near the amino terminus of human TRAF6. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys192 of human TRAF2 or in the central region within TRAF3. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

    Background

    TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.
    While TRAF2 was originally described through its interaction with TNFRII, it has since been shown to interact with other surface receptors including CD27, CD30, CD40, 4-1BB, Ox40, HVEM/ATAR, and LMP-1 (1-3). TRAF2 also associates with a large number of intracellular proteins, including TRADD, FADD, I-TRAF/TANK, TRIP, A20, c-IAP1 and 2, Casper, RIP, and NIK, which help to regulate cell survival. Dominant negative and knockout studies have shown that TRAF2 plays an important role in TNF-mediated activation of NF-κB and the MAPK/JNK kinase pathway (5-7).

    TRAF6 plays a critical role in innate and adaptive immunity, bone metabolism, and development of certain tissues including the nervous system (8). TRAF6 deficiency results in osteopetrosis and defective IL-1, CD40, and LPS signaling (9) as well as defects in neuronal development (10). Unlike other TRAF family members that mediate signaling through TNF, TRAF6 has unique binding activities (11) that result in signaling responses from the interleukin-1 receptor (IL-1R) (12), toll-like receptor (13,14), CD (15), RANK (16,17), and p75 neurotrophin receptor (18). TRAF6 associates directly with CD40 and RANK, and indirectly with IL-1R/TLR through IRAK (13). This leads to activation of NF-κB and MAP kinase signaling pathways through downstream association with the TAB/TAK-1 complex (19). TRAF6 also activates Src family nonreceptor tyrosine kinases leading to Akt activation (20).
    1. Arch, R.H. et al. (1998) Genes Dev 12, 2821-30.
    2. Chung, J.Y. et al. (2002) J Cell Sci 115, 679-88.
    3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91.
    4. Rothe, M. et al. (1994) Cell 78, 681-92.
    5. Yeh, W.C. et al. (1997) Immunity 7, 715-25.
    6. Reinhard, C. et al. (1997) EMBO J 16, 1080-92.
    7. Rothe, M. et al. (1995) Science 269, 1424-7.
    8. Wu, H. and Arron, J.R. (2003) Bioessays 25, 1096-105.
    9. Lomaga, M.A. et al. (1999) Genes Dev 13, 1015-24.
    10. Lomaga, M.A. et al. (2000) J Neurosci 20, 7384-93.
    11. Ye, H. et al. (2002) Nature 418, 443-7.
    12. Cao, Z. et al. (1996) Nature 383, 443-6.
    13. Muzio, M. et al. (1997) Science 278, 1612-5.
    14. Medzhitov, R. et al. (1998) Mol Cell 2, 253-8.
    15. Ishida, T. et al. (1996) J Biol Chem 271, 28745-8.
    16. Darnay, B.G. et al. (1998) J Biol Chem 273, 20551-5.
    17. Wong, B.R. et al. (1998) J Biol Chem 273, 28355-9.
    18. Khursigara, G. et al. (1999) J Biol Chem 274, 2597-600.
    19. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6.
    20. Wong, B.R. et al. (1999) Mol Cell 4, 1041-9.

    Database Links

    UniProt ID: Q13077 , Q9Y4K3 , Q13114 , Q12933


    Entrez-Gene Id: 7185 , 7189 , 7187 , 7186


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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