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Render Timestamp: 2024-11-22T11:41:11.286Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-10-10 13:16:11.434
Product last modified at: 2024-11-07T12:15:15.273Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. TRAF6 (E2K9D) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TRAF6 (E2K9D) Rabbit mAb #67591

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TRAF6 (E2K9D) Rabbit mAb recognizes endogenous levels of total TRAF6 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to a central region of mouse TRAF6 protein.

    Background

    TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.
    TRAF6 plays a critical role in innate and adaptive immunity, bone metabolism, and development of certain tissues including the nervous system (5). TRAF6 deficiency results in osteopetrosis and defective IL-1, CD40, and LPS signaling (6) as well as defects in neuronal development (7). Unlike other TRAF family members that mediate signaling through TNF, TRAF6 has unique binding activities (8) that result in signaling responses from the interleukin-1 receptor (IL-1R) (9), toll-like receptor (10,11), CD40 (12), RANK (13,14), and p75 neurotrophin receptor (15). TRAF6 associates directly with CD40 and RANK, and indirectly with IL-1R/TLR through IRAK (10). This leads to activation of NF-κB and MAP kinase signaling pathways through downstream association with the TAB/TAK-1 complex (16). TRAF6 also activates Src family nonreceptor tyrosine kinases leading to Akt activation (17).
    1. Arch, R.H. et al. (1998) Genes Dev 12, 2821-30.
    2. Chung, J.Y. et al. (2002) J Cell Sci 115, 679-88.
    3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91.
    4. Rothe, M. et al. (1994) Cell 78, 681-92.
    5. Wu, H. and Arron, J.R. (2003) Bioessays 25, 1096-105.
    6. Lomaga, M.A. et al. (1999) Genes Dev 13, 1015-24.
    7. Lomaga, M.A. et al. (2000) J Neurosci 20, 7384-93.
    8. Ye, H. et al. (2002) Nature 418, 443-7.
    9. Cao, Z. et al. (1996) Nature 383, 443-6.
    10. Muzio, M. et al. (1997) Science 278, 1612-5.
    11. Medzhitov, R. et al. (1998) Mol Cell 2, 253-8.
    12. Ishida, T. et al. (1996) J Biol Chem 271, 28745-8.
    13. Darnay, B.G. et al. (1998) J Biol Chem 273, 20551-5.
    14. Wong, B.R. et al. (1998) J Biol Chem 273, 28355-9.
    15. Khursigara, G. et al. (1999) J Biol Chem 274, 2597-600.
    16. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6.
    17. Wong, B.R. et al. (1999) Mol Cell 4, 1041-9.

    Database Links

    UniProt ID: P70196


    Entrez-Gene Id: 22034


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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